actinomycin in a sentence
1) Actinomycin is a complex molecule that intercalates DNA and prevents RNA synthesis.
2) For these reasons we intend to perform future studies on DNA fragments containing isolated actinomycin binding sites.
3) This footprint contains three potential overlapping actinomycin binding sites which must therefore be characterised by different dissociation parameters.
actinomycin example sentences
4) Figure 1 presents the time course of dissociation of actinomycin from tyr T DNA measured at three different temperatures.5) We found that protein synthesis in sea urchin embryos treated with actinomycin D was considerably less than in untreated embryos.
6) This unequal division process is dependent on actinomycin , and by the end of stage 3 the AB cell divides.
7) We have investigated the kinetics of dissociation of actinomycin D from DNA by a variation of the footprinting technique.
8) The experiment with actinomycin suggests that for the first 3 hours or so LTP does not depend on gene transcription.
9) Actinomycin produces four clear regions of protection each of which are centred around one or more of the 9 GpC sites.
10) Similarly our own footprinting studies have suggested that actinomycin binding can be potentiated by flanking regions of (GT) n .
11) Actinomycin D and distamycin were purchased from Sigma and stored as solutions in 10mM Tris-HCl pH 8.0 containing 10mM NaCl.
12) Two plates of Bud8 were left untreated, and two plates were treated with actinomycin D for ∼2 h. Samples were then harvested and analyzed as for HepG2 cells.
13) Compared to controls it was found that the use of the actinomycin D did inhibit the protective effects of Arsenicum album 30C and 200C against arsenic toxicity.
14) Other lanes show the time (in minutes) after addition of unlabelled calf thymus DNA to a complex containing 20μM actinomycin .
15) We estimate that the dissociation time for distamycin at 4°C (10 s) must be at least 500 times faster than that of actinomycin under similar conditions.
use actinomycin in a sentence
16) The faster reappearance of bands at the upper (5') side of this footprint therefore suggests that actinomycin dissociates from CGCG faster than CGCT.17) The upper part of the footprint at sites 7 and 8 reappears faster than the lower portion, suggesting that actinomycin dissociates from site 8 faster than site 7.
18) Secondly when potential actinomycin binding sites are in close proximity (for example in GCGC) only one of the potential sites can be occupied at a time.
19) Ptyr 2 DNA contains only one such site, position 6 (TGCC), from which actinomycin does indeed dissociate more rapidly than the other sites.
20) The studies with tyr T and ptyr 2 DNA suggest that actinomycin dissociates from each of its GC binding sites with rates that vary according to the surrounding sequences.
21) The last lane shows the footprinting pattern obtained when the radiolabelled DNA fragment is mixed with the unlabelled calf thymus DNA before adding actinomycin .
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